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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Annexin A13 Polyclonal antibody (25153-1-AP)

    WB analysis using 25153-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using Annexin A13 Polyclonal antibody (25153-1-AP)

    WB analysis of mouse small intestine using 25153-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 25153-1-AP (Annexin A13 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Annexin A13 Polyclonal antibody (25153-1-AP)

    IHC staining of human small intestine using 25153-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using Annexin A13 Polyclonal antibody (25153-1-AP)

    IHC staining of human small intestine using 25153-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 25153-1-AP (Annexin A13 antibody at dilution of 1:50 (under 40x lens).

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Annexin A13 Polyclonal antibody

Annexin A13 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25153-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ANXA13, Annexin 13, Annexin XIII, Annexin-13, ANX13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 600 ug/ml
20 μL, 600 ug/ml
150 μL, 600 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse small intestine tissue, rat small intestine tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

25153-1-AP targets Annexin A13 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Annexin A13 fusion protein Ag18581 相同性解析による交差性が予測される生物種
Full Name annexin A13
Calculated molecular weight 316 aa, 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC125158
Gene Symbol Annexin A13
Gene ID (NCBI) 312
RRIDAB_2879928
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP27216
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Annexin A13 (ANXA13) is believed to be the original founder gene of the 12-member vertebrate annexin A family, and it has acquired an intestine-specific expression associated with a highly differentiated intracellular transport function (PMID: 11961095). ANXA13, which is highly expressed in the digestive system and believed to be the original founder gene of the annexin A family (PMID: 28423508). Limited evidence suggests that ANXA13 may play a role in apoptotic cell-mediated immunosuppression, hypertension, and cell death recognition (PMID: 28423508). ANXA13 is highly expressed in the human colon adenocarcinoma cell line HT29.

Protocols

Product Specific Protocols
WB protocol for Annexin A13 antibody 25153-1-APDownload protocol
IHC protocol for Annexin A13 antibody 25153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Med Chem

Discovery of a Novel 1,4-Benzodiazepine Derivative as a Highly Selective ANXA3 Degrader for the Treatment of Triple-Negative Breast Cancer

Authors - Kunlin Liu
View Article