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Validation Data Gallery

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  • Western Blot (WB) analysis of pig stomach tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    WB analysis of pig stomach using 66869-1-Ig (same clone as 66869-1-PBS)

    pig stomach tissue were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-29 cells using Annexin A10 Monoclonal antibody (66869-1-Ig)

    WB analysis of HT-29 using 66869-1-Ig (same clone as 66869-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66869-1-Ig (Annexin A10 antibody) at dilution of 1:2100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of human stomach using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of human stomach using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of human colon cancer using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of human colon cancer using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of human pancreas cancer using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of human pancreas cancer using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of human colon using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of human colon using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of mouse pancreas using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IHC staining of mouse pancreas using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66869-1-Ig (Annexin A10 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IF Staining of human colon cancer using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human colon cancer tissue using Annexin A10 Monoclonal antibody (66869-1-Ig)

    IF Staining of human colon cancer using 66869-1-Ig (same clone as 66869-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Annexin A10 antibody (66869-1-Ig, Clone: 1A7A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66869-1-PBS in a different storage buffer formulation.

Annexin A10 Monoclonal antibody, PBS Only

Annexin A10 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66869-1-PBS
Clone No.1A7A11

Host / Isotype

Mouse / IgG2a

Reactivity

Human, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Annexin 10, Annexin 14, annexin A10, ANX14, ANXA10

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66869-1-PBS targets Annexin A10 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, pig, mouse samples.

Tested Reactivity Human, pig, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27098

Product name: Recombinant human ANXA10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 160-324 aa of BC007320

Sequence: QGTREEGYTDPAMAAQDAMVLWEACQQKTGEHKTMLQMILCNKSYQQLRLVFQEFQNISGQDMVDAINECYDGYFQELLVAIVLCVRDKPAYFAYRLYSAIHDFGFHNKTVIRILIARSEIDLLTIRKRYKERYGKSLFHDIRNFASGHYKKALLAICAGDAEDY

相同性解析による交差性が予測される生物種
Full Name annexin A10
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC007320
Gene Symbol ANXA10
Gene ID (NCBI) 11199
RRIDAB_2882206
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UJ72
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ANXA10, member of the Annexin family, is a calcium-and-phospholipid-binding protein. It is implicated in multiple physiological processes, including growth regulation, cell division, apoptosis, and differentiation. ANXA10 was recently observed to be upregulated in Barrett's esophagus, oral cancers, pancreatic cancer and colon cancer.