Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, rat kidney tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51109-1-AP targets Angptl4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Angptl4 fusion protein Ag0613 相同性解析による交差性が予測される生物種 |
| Full Name | angiopoietin-like 4 |
| Calculated molecular weight | 405 aa, 45 kDa |
| GenBank accession number | BC078944 |
| Gene Symbol | Angptl4 |
| Gene ID (NCBI) | 362850 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6TMA8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Angiopoietin-like protein 4 (ANGPTL4), a member of the angiopoietin-like protein subfamily, is a secretory protein that functions as an important modulator of glucose and lipid metabolism. ANGPTL4 inhibits lipoprotein lipase (LPL)-dependent lipolysis thereby limiting the uptake of free fatty acids. Overexpression of ANGPTL4 results in hypertriglyceridemia, while ANGPTL4 deficiency suppresses foam formation in macrophages and protects against atherosclerosis. ANGPTL4 may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. ANGPTL4 has three functional domains: the signal peptide, the coiled-coil (N-terminal chain, CCD), and the fibrinogen-like (C-terminal chain, FLD) domains. ANGPTL4 is a glycosylated secreted protein (fANGPTL4, 45-65 kDa). fANGPTL is then rapidly post-translationally cleaved into an N-terminal coiled-coil domain (nANGPTL4, ~ 15 kDa) and a C-terminal fibrinogen-like domain (cANGPTL4, ~ 35 kDa)(PMID: 37300585 ).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Angptl4 antibody 51109-1-AP | Download protocol |
| IHC protocol for Angptl4 antibody 51109-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |