• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Angiopoietin-2 Recombinant antibody (83816-1-RR)

    WB analysis using 83816-1-RR (same clone as 83816-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using Angiopoietin-2 Recombinant antibody (83816-1-RR)

    IP experiment of mouse lung using 83816-1-RR (same clone as 83816-1-PBS)

    IP result of anti-Angiopoietin-2 (IP:83816-1-RR, 4ug; Detection:83816-1-RR 1:1500) with mouse lung tissue lysate 1200 ug. This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Angiopoietin-2 Recombinant antibody (83816-1-RR)

    IHC staining of mouse kidney using 83816-1-RR (same clone as 83816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using Angiopoietin-2 Recombinant antibody (83816-1-RR)

    IHC staining of mouse kidney using 83816-1-RR (same clone as 83816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Angiopoietin-2 Recombinant antibody (83816-1-RR)

    IHC staining of mouse brain using 83816-1-RR (same clone as 83816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Angiopoietin-2 Recombinant antibody (83816-1-RR)

    IHC staining of mouse brain using 83816-1-RR (same clone as 83816-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83816-1-RR (Angiopoietin-2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83816-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83816-1-RR

    Affinity and Kinetic Characterization of 83816-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83816-1-RR against Mouse Angiopoietin-2 were performed. The affinity constant is below 1 pM.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Mouse Angiopoietin-2 Recombinant antibody, PBS Only

Angiopoietin-2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 83816-1-PBS
Clone No.240760D5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IP, ELISA

Synonyms

Angpt2, angiopoietin 2, Ang2, Ang 2, Agpt2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83816-1-PBS targets Angiopoietin-2 in WB, IHC, IP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name angiopoietin 2
Calculated molecular weight56KD
Observed molecular weight 68kDa
GenBank accession numberNM_007426.4
Gene Symbol Angpt2
Gene ID (NCBI) 11601
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO35608
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).