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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Angiopoietin-2 Polyclonal antibody (31499-1-AP)

    WB analysis using 31499-1-AP (same clone as 31499-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Angiopoietin-2 Polyclonal antibody (31499-1-AP)

    WB analysis using 31499-1-AP (same clone as 31499-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat lung tissue using Angiopoietin-2 Polyclonal antibody (31499-1-AP)

    IP experiment of rat lung using 31499-1-AP (same clone as 31499-1-PBS)

    IP result of anti-Angiopoietin-2 (IP:31499-1-AP, 4ug; Detection:31499-1-AP 1:3000) with rat lung tissue lysate 1200 ug. This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Angiopoietin-2 Polyclonal antibody (31499-1-AP)

    IHC staining of mouse brain using 31499-1-AP (same clone as 31499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Angiopoietin-2 Polyclonal antibody (31499-1-AP)

    IHC staining of mouse colon using 31499-1-AP (same clone as 31499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Angiopoietin-2 Polyclonal antibody (31499-1-AP)

    IHC staining of mouse colon using 31499-1-AP (same clone as 31499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Angiopoietin-2 Polyclonal antibody (31499-1-AP)

    IHC staining of mouse brain using 31499-1-AP (same clone as 31499-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31499-1-AP (Angiopoietin-2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31499-1-PBS in a different storage buffer formulation.

Mouse Angiopoietin-2 Polyclonal antibody, PBS Only

Angiopoietin-2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 31499-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Angpt2, Agpt2, Ang 2, Ang2, angiopoietin 2

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31499-1-PBS targets Angiopoietin-2 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Eg0666

Product name: recombinant mouse Angiopoietin-2 protein

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 68-496 aa of NM_007426.4

Sequence: DAPLDYDDSVQRLQVLENILENNTQWLMKLENYIQDNMKKEMVEIQQNVVQNQTAVMIEIGTSLLNQTAAQTRKLTDVEAQVLNQTTRLELQLLQHSISTNKLEKQILDQTSEINKLQNKNSFLEQKVLDMEGKHSEQLQSMKEQKDELQVLVSKQSSVIDELEKKLVTATVNNSLLQKQQHDLMETVNSLLTMMSSPNSKSSVAIRKEEQTTFRDCAEIFKSGLTTSGIYTLTFPNSTEEIKAYCDMDVGGGGWTVIQHREDGSVDFQRTWKEYKEGFGSPLGEYWLGNEFVSQLTGQHRYVLKIQLKDWEGNEAHSLYDHFYLAGEESNYRIHLTGLTGTAGKISSISQPGSDFSTKDSDNDKCICKCSQMLSGGWWFDACGPSNLNGQYYPQKQNTNKFNGIKWYYWKGSGYSLKATTMMIRPADF

相同性解析による交差性が予測される生物種
Full Name angiopoietin 2
Calculated molecular weight56KD
Observed molecular weight 68 kDa
GenBank accession numberNM_007426.4
Gene Symbol Angpt2
Gene ID (NCBI) 11601
RRIDAB_3742440
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDO35608
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Angiopoietin-2 (ANGPT2, ANG-2) is as a regulator of vessel enlargement, and ANGPT2 inhibition prevented pathological vessel enlargement (PMID: 34758631). ANGPT2 destabilizes vessels and promotes angiogenesis through antagonism of ANGPT1 (PMID: 34758631). Ang2 was initially identified by homology with Ang1 and is expressed predominantly by endothelial cells, where it is stored in intracellular secretory granules called Weibel-Palade bodies (WPB) and promptly released after endothelium activation. Like Ang1, Ang2 binds to Tie2 receptor, but with different pathophysiological effects. While Ang1 fosters endothelial stabilization, Ang2 can antagonize Ang1, blocking Tie2 activation and leading to vessel destabilization (PMID: 31756341).

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