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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse pancreas tissue using Amylase Alpha Polyclonal antibody (12540-1-AP)

    WB analysis of mouse pancreas using 12540-1-AP (same clone as 12540-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12540-1-AP (Amylase, alpha antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Amylase Alpha Polyclonal antibody (12540-1-AP)

    IHC staining of human pancreas cancer using 12540-1-AP (same clone as 12540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Amylase Alpha Polyclonal antibody (12540-1-AP)

    IHC staining of human pancreas cancer using 12540-1-AP (same clone as 12540-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12540-1-AP (Amylase, alpha antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat pancreas tissue using Amylase Alpha Polyclonal antibody (12540-1-AP)

    IF Staining of rat pancreas using 12540-1-AP (same clone as 12540-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat pancreas tissue using Amylase Alpha antibody (12540-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12540-1-PBS in a different storage buffer formulation.

Amylase Alpha Polyclonal antibody, PBS Only

Amylase Alpha Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 12540-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

AMY2B, Amylase, alpha, 1,4-alpha-D-glucan glucanohydrolase 2B, Alpha amylase, Alpha amylase 2B

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12540-1-PBS targets Amylase Alpha in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3260

Product name: Recombinant human AMY2B protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 160-511 aa of BC011179

Sequence: SGDIENYNDATQVRDCRLVGLLDLALEKDYVRSKIAEYMNHLIDIGVAGFRLDASKHMWPGDIKAILDKLHNLNSNWFPAGSKPFIYQEVIDLGGEPIKSSDYFGNGRVTEFKYGAKLGTVIRKWNGEKMSYLKNWGEGWGFMPSDRALVFVDNHDNQRGHGAGGASILTFWDARLYKMAVGFMLAHPYGFTRVMSSYRWPRQFQNGNDVNDWVGPPNNNGVIKEVTINPDTTCGNDWVCEHRWRQIRNMVNFRNVVDGQPFTNWYDNGSNQVAFGRGNRGFIVFNNDDWTFSLTLQTGLPAGTYCDVISGDKINGNCTGIKIYVSDDGKAHFSISNSAEDPFIAIHAESKL

相同性解析による交差性が予測される生物種
Full Name amylase, alpha 2B (pancreatic)
Calculated molecular weight 511 aa, 58 kDa
Observed molecular weight 58 kDa
GenBank accession numberBC011179
Gene Symbol AMY2B
Gene ID (NCBI) 280
RRIDAB_2273990
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP19961
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AMY2B(alpha-amylase 2B) belongs to the glycosyl hydrolase 13 family. This protein is involved in the endohydrolysis of 1,4-alpha-glucosidic linkages in oligosaccharides and polysaccharides. The full length 58 kDa protein has a signal peptide with 15 amino acids. Human amylases (Amy1, Amy2A, and Amy2B) commonly have two potential N-glycosylation sites (N427 and N476) in their C-terminal region (S Takashima, J Amano, 2012). 12540-1-AP can recognize both AMY2A and AMY2B.

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