Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis of MCF-7 using 16036-1-AP (same clone as 16036-1-PBS)
MCF-7 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 16036-1-AP (same clone as 16036-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 16036-1-AP (Amphiregulin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IP experiment of HepG2 using 16036-1-AP (same clone as 16036-1-PBS)
IP result of anti-Amphiregulin (IP:16036-1-AP, 4ug; Detection:16036-1-AP 1:2000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16036-1-AP (Amphiregulin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 16036-1-AP (same clone as 16036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 16036-1-AP (Amphiregulin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 16036-1-AP (same clone as 16036-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16036-1-AP (Amphiregulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IF Staining of mouse embryo using 16036-1-AP (same clone as 16036-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse embryo tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 16036-1-AP (same clone as 16036-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IF Staining of mouse embryo using 16036-1-AP (same clone as 16036-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse embryo tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Amphiregulin antibody (16036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 16036-1-AP (same clone as 16036-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Amphiregulin antibody (16036-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
FC experiment of HeLa using 16036-1-AP (same clone as 16036-1-PBS)
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Amphiregulin (16036-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.
×
1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human Amphiregulin (16036-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 16036-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Amphiregulin Polyclonal antibody, PBS Only

Amphiregulin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Cat No. 16036-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, FC (Intra), IP, ELISA

Synonyms

AREG, AR, AREGB, Colorectum cell-derived growth factor, CRDGF

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16036-1-PBS targets Amphiregulin in WB, IHC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Amphiregulin fusion protein Ag8907 相同性解析による交差性が予測される生物種
Full Name	amphiregulin
Calculated molecular weight	252 aa, 28 kDa
Observed molecular weight	43 kDa
GenBank accession number	BC009799
Gene Symbol	Amphiregulin/AREG
Gene ID (NCBI)	374
RRID	AB_2227602
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15514
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

Amphiregulin (AREG) is one of the ligands of the epidermal growth factor receptor (EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms (PMID: 9642297).

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of MCF-7 using 16036-1-AP (same clone as 16036-1-PBS)

WB analysis of A549 using 16036-1-AP (same clone as 16036-1-PBS)

IP experiment of HepG2 using 16036-1-AP (same clone as 16036-1-PBS)

IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

IHC staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)

IHC staining of human stomach cancer using 16036-1-AP (same clone as 16036-1-PBS)

IHC staining of human colon using 16036-1-AP (same clone as 16036-1-PBS)

IF Staining of mouse embryo using 16036-1-AP (same clone as 16036-1-PBS)

IF Staining of human placenta using 16036-1-AP (same clone as 16036-1-PBS)

IF Staining of human colon cancer using 16036-1-AP (same clone as 16036-1-PBS)