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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using Amphiphysin Polyclonal antibody (13379-1-AP)

    WB analysis of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse lung tissue using Amphiphysin Polyclonal antibody (13379-1-AP)

    WB analysis of mouse lung using 13379-1-AP (same clone as 13379-1-PBS)

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13379-1-AP (Amphiphysin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using Amphiphysin Polyclonal antibody (13379-1-AP)

    IP experiment of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

    IP result of anti-Amphiphysin (IP:13379-1-AP, 4ug; Detection:13379-1-AP 1:1500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Amphiphysin Polyclonal antibody (13379-1-AP)

    IHC staining of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Amphiphysin Polyclonal antibody (13379-1-AP)

    IHC staining of mouse brain using 13379-1-AP (same clone as 13379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Amphiphysin Polyclonal antibody (13379-1-AP)

    IHC staining of mouse cerebellum using 13379-1-AP (same clone as 13379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Amphiphysin Polyclonal antibody (13379-1-AP)

    IHC staining of mouse cerebellum using 13379-1-AP (same clone as 13379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13379-1-AP (Amphiphysin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Amphiphysin Polyclonal antibody (13379-1-AP)

    IHC staining of human pancreas cancer using 13379-1-AP (same clone as 13379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using Amphiphysin Polyclonal antibody (13379-1-AP)

    IHC staining of human pancreas cancer using 13379-1-AP (same clone as 13379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13379-1-AP (Amphiphysin antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13379-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Amphiphysin Polyclonal antibody, PBS Only

Amphiphysin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 13379-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13379-1-PBS targets Amphiphysin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag4204

Product name: Recombinant human AMPH protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 346-695 aa of BC034376

Sequence: KKEETLLDLDFDPFKPEVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQPQDTSLFTMQTDQSMICNLAESEQAPPTEPKAEEPLAAVTPAVGLDLGMDTRAEEPVEEAVIIPGADADAAVGTLVSAAEGAPGEEAEAETATVPAGEGVSLEEAKIGTETTEGAESAQPEAEELEATVPQEKVIPSVVIEPASNHEEEGENEITIGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPAMGAADQLASAREASQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLD

相同性解析による交差性が予測される生物種
Full Name amphiphysin
Calculated molecular weight 695 aa, 76 kDa
Observed molecular weight 115-125 kDa
GenBank accession numberBC034376
Gene Symbol AMPH
Gene ID (NCBI) 273
RRIDAB_2226789
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49418
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Amphiphysin (AMPH), a synaptic vesicle-associated protein that is highly concentrated in nerve terminal, is an autoantigen of Stiff-Man syndrome with breast cancer. Amphiphysin contains a N-terminal BAR (Bin1/Amphiphysin/Rv167) domain followed by an endocytosis domain, and a C-terminal SH3 domain. Amphiphysin has been proposed to function as a linker between the clathrin coat and dynamin in the endocytosis of synaptic vesicles. It may also participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. (PMID: 8552632; 8245793; 10559861)

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