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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Alix Recombinant monoclonal antibody (86644-1-RR)

    WB analysis using 86644-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86644-1-RR (ALIX; AIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 86644-1-RR

    Affinity and Kinetic Characterization of 86644-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86644-1-RR against Human Alix were performed. The affinity constant is 6.12 nM.

Alix Recombinant monoclonal antibody

Alix Uni-rAb® Recombinant Antibody for WB, ELISA
Cat No. 86644-1-RR
Clone No.251574C12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

PDCD6IP, AIP1, ALG-2-interacting protein 1, ALG-2-interacting protein X, DRIP4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293T cells, PANC-1 cells, Jurkat cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86644-1-RR targets Alix in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Alix fusion protein Ag3074 相同性解析による交差性が予測される生物種
Full Name programmed cell death 6 interacting protein
Calculated molecular weight 868 aa, 96 kDa
Observed molecular weight100 kDa
GenBank accession numberBC020066
Gene Symbol Alix
Gene ID (NCBI) 10015
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8WUM4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.

Protocols

Product Specific Protocols
WB protocol for Alix antibody 86644-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols