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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Albumin Recombinant antibody (82783-6-RR)

    WB analysis using 82783-6-RR (same clone as 82783-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82783-6-RR (Albumin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Hepatocellular cancer using Albumin Recombinant antibody (82783-6-RR)

    IHC staining of Human Hepatocellular cancer using 82783-6-RR (same clone as 82783-6-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 82783-6-RR (Albumin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Albumin Recombinant antibody (82783-6-RR)

    IHC staining of human stomach using 82783-6-RR (same clone as 82783-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82783-6-RR (Albumin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using Albumin Recombinant antibody (82783-6-RR)

    IHC staining of human stomach using 82783-6-RR (same clone as 82783-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 82783-6-RR (Albumin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Human Hepatocellular cancer using Albumin Recombinant antibody (82783-6-RR)

    IHC staining of Human Hepatocellular cancer using 82783-6-RR (same clone as 82783-6-PBS)

    Immunohistochemical analysis of paraffin-embedded Human Hepatocellular cancer slide using 82783-6-RR (Albumin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Recombinant antibody (82783-6-RR)

    IF Staining of mouse liver using 82783-6-RR (same clone as 82783-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Recombinant antibody (82783-6-RR)

    IF Staining of mouse liver using 82783-6-RR (same clone as 82783-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Albumin Recombinant antibody (82783-6-RR)

    IF Staining of HepG2 using 82783-6-RR (same clone as 82783-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Albumin antibody (82783-6-RR, Clone: 4K1 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82783-6-PBS in a different storage buffer formulation.

Albumin Recombinant monoclonal antibody, PBS Only

Albumin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 82783-6-PBS
Clone No.4K1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

ALB, 4K1, HSA, Serum albumin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82783-6-PBS targets Albumin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag9569

Product name: Recombinant human Albumin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 256-609 aa of BC034023

Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL

相同性解析による交差性が予測される生物種
Full Name albumin
Calculated molecular weight 609 aa, 69 kDa
Observed molecular weight66 kDa
GenBank accession numberBC034023
Gene Symbol Albumin
Gene ID (NCBI) 213
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP02768
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. (21388516, 23832071)

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