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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AlaRS Monoclonal antibody (67909-1-Ig)

    WB analysis using 67909-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AlaRS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • Western Blot (WB) analysis of HeLa cells using AlaRS Monoclonal antibody (67909-1-Ig)

    WB analysis of HeLa using 67909-1-Ig

    WB result of AlaRS antibody (67909-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AlaRS transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using AlaRS Monoclonal antibody (67909-1-Ig)

    WB analysis using 67909-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67909-1-Ig (AARS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using AlaRS Monoclonal antibody (67909-1-Ig)

    IF Staining of mouse liver using 67909-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using AlaRS Monoclonal antibody (67909-1-Ig)

    IF Staining of NIH/3T3 using 67909-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AlaRS Monoclonal antibody (67909-1-Ig)

    IF Staining of HepG2 using 67909-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using AlaRS Monoclonal antibody (67909-1-Ig)

    IF Staining of A431 using 67909-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using AlaRS antibody (67909-1-Ig, Clone: 1C8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO Validated

AlaRS Monoclonal antibody

AlaRS Monoclonal Antibody for WB, IF/ICC, IF-P, ELISA
Cat No. 67909-1-Ig
Clone No.1C8H3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, ELISA

Synonyms

AARS, 1C8H3, AARS1, Alanine--tRNA ligase, cytoplasmic, Alanyl-tRNA synthetase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inHepG2 cells, A431 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67909-1-Ig targets AlaRS in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11151

Product name: Recombinant human AARS protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC011451

Sequence: MDSTLTASEIRQRFIDFFKRNEHTYVHSSATIPLDDPTLLFANAGMNQFKPIFLNTIDPSHPMAKLSRAANTQKCIRAGGKHNDLDDVGKDVYHHTFFEMLGSWSFGDYFKELACKMALELLTQEFGIPIERLYVTYFGGDEAAGLEADLECKQIWQNLGLDDTKILPGNMKDNFWEMGDTGPCGPCSEIHYDRIGGRDAAHLVNQDDPNVLEIWNLVFIQYNREADGILKPLPKKSIDTGMGLERLVSVLQNKMSNYDTDLFVPYFEAIQKGTGARPYTGKVGAEDADGIDMAYRVLADHARTITVALADGGRPDNTGRGYVLRRILRRAVRYAHEKLNASRGFFATLV

相同性解析による交差性が予測される生物種
Full Name alanyl-tRNA synthetase
Calculated molecular weight 968 aa, 107 kDa
Observed molecular weight107 kDa
GenBank accession numberBC011451
Gene Symbol AlaRS
Gene ID (NCBI) 16
RRIDAB_2918664
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP49588
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AARS(alanyl-tRNA synthetase) is also named as AlaRS(alanine tRNA ligase 1, cytoplasmic), renal carcinoma antigen NY-REN-42 and belongs to the class-II aminoacyl-tRNA synthetase family. It can interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. AARS consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs(PMID:19661429).

Protocols

Product Specific Protocols
IF protocol for AlaRS antibody 67909-1-IgDownload protocol
WB protocol for AlaRS antibody 67909-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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