Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83367-2-RR targets Afp in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | alpha fetoprotein |
| Calculated molecular weight | 67KD |
| Observed molecular weight | 67-72 kDa |
| GenBank accession number | NM_007423.4 |
| Gene Symbol | Afp |
| Gene ID (NCBI) | 11576 |
| RRID | AB_3671021 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02772 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Alpha-fetoprotein (AFP) is one of the earliest proteins to be synthesised by the embryonic liver. The synthesis of AFP decreases dramatically after birth and only trace amounts are expressed in the adult liver. High serum AFP concentrations are also found in connection with primary liver cancer and teratocarcinoma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Afp antibody 83367-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |