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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Afp Polyclonal antibody (31256-1-AP)

    WB analysis using 31256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31256-1-AP (Afp antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Afp Polyclonal antibody (31256-1-AP)

    WB analysis using 31256-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31256-1-AP (Afp antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using AFP Polyclonal antibody (31256-1-AP)

    IHC staining of mouse placenta using 31256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 31256-1-AP (AFP antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse placenta tissue using AFP Polyclonal antibody (31256-1-AP)

    IHC staining of mouse placenta using 31256-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 31256-1-AP (AFP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Afp Polyclonal antibody (31256-1-AP)

    IF Staining of HepG2 using 31256-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Afp antibody (31256-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

AFP Polyclonal antibody

AFP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 31256-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

alpha fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse placenta tissue, HepG2 cells, human placenta tissue
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31256-1-AP targets AFP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFP fusion protein Eg0670 相同性解析による交差性が予測される生物種
Full Name alpha fetoprotein
Calculated molecular weight67KD
Observed molecular weight68-72 kDa
GenBank accession numberNM_007423.4
Gene Symbol Afp
Gene ID (NCBI) 11576
RRIDAB_3669920
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP02772
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-fetoprotein (AFP) is one of the earliest proteins to be synthesised by the embryonic liver. The synthesis of AFP decreases dramatically after birth and only trace amounts are expressed in the adult liver. High serum AFP concentrations are also found in connection with primary liver cancer and teratocarcinoma.

Protocols

Product Specific Protocols
WB protocol for AFP antibody 31256-1-APDownload protocol
IHC protocol for AFP antibody 31256-1-APDownload protocol
IF protocol for AFP antibody 31256-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols