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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using mouse Adiponectin Polyclonal antibody (30723-1-AP)

    WB analysis using 30723-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30723-1-AP (Adiponectin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using Adiponectin Polyclonal antibody (30723-1-AP)

    IHC staining of mouse brown adipose using 30723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 30723-1-AP (Adiponectin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using Adiponectin Polyclonal antibody (30723-1-AP)

    IHC staining of mouse brown adipose using 30723-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 30723-1-AP (Adiponectin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Adiponectin Polyclonal antibody

Adiponectin Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30723-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Adipoq, apM1, adipo, Acrp30

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse adipose tissue, mouse serum
Positive IHC detected inmouse brown adipose tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30723-1-AP targets Adiponectin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name adiponectin, C1Q and collagen domain containing
Calculated molecular weight27kd
Observed molecular weight 28 kDa
GenBank accession numberNM_009605
Gene Symbol Adiponectin
Gene ID (NCBI) 11450
RRIDAB_3669755
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ60994
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Protocols

Product Specific Protocols
WB protocol for Adiponectin antibody 30723-1-APDownload protocol
IHC protocol for Adiponectin antibody 30723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols