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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    WB analysis using 20332-1-AP (same clone as 20332-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20332-1-AP (Adenosine A1 Receptor antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    IHC staining of mouse brain using 20332-1-AP (same clone as 20332-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    IHC staining of mouse brain using 20332-1-AP (same clone as 20332-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    IHC staining of mouse cerebellum using 20332-1-AP (same clone as 20332-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    IHC staining of mouse cerebellum using 20332-1-AP (same clone as 20332-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20332-1-AP (Adenosine A1 Receptor antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using Adenosine A1 Receptor Polyclonal antibody (20332-1-AP)

    FC experiment of SH-SY5Y using 20332-1-AP (same clone as 20332-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Adenosine A1 Receptor Polyclonal antibody (20332-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 20332-1-PBS in a different storage buffer formulation.

Adenosine A1 Receptor Polyclonal antibody, PBS Only

Adenosine A1 Receptor Polyclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 20332-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Adenosine receptor A1, ADORA1, RDC7

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20332-1-PBS targets Adenosine A1 Receptor in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14092

Product name: Recombinant human ADORA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 184-243 aa of BC026340

Sequence: NFFVWVLPPLLLMVLIYLEVFYLIRKQLNKKVSASSGDPQKYYGKELKIAKSLALILFLF

相同性解析による交差性が予測される生物種
Full Name adenosine A1 receptor
Calculated molecular weight 326 aa, 37 kDa
Observed molecular weight40 and 45 kDa
GenBank accession numberBC026340
Gene Symbol Adenosine A1 Receptor
Gene ID (NCBI) 134
RRIDAB_2878673
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP30542
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ADORA1, also named as RDC7, is a receptor for adenosine. It is mediated by G proteins which inhibit adenylyl cyclase. Adenosine is an important mediator of ethanol intoxication and exerts some of its effects via ADORA1 in the central nervous system.

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