Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.
| Description | The Actin-Chromobody allows imaging of actin dynamics in real-time in living cells.
• Easily transfect your cells and non-invasively visualize actin cytoskeleton in real-time • Target and trace microfilaments in your cell line of interest • Use Actin-Chromobody as a marker of cellular morphology and as a probe for cellular motility • Actin-Chromobody has no notable effect cell viability or migration |
| Applications | Live cell imaging Visualizing actin dynamics Actin as biomarker or control |
| Specificity | Actin microfilaments in human, rodent and plant cells, zebrafish, Toxoplasma gondii |
| Reporter | TagRFP |
| Vector type | Mammalian expression vector |
| Promoter | Constitutive CMV IE |
| Codon usage | Mammalian |
| Selection | Kan/Neo |
| Nuclear localization signal (NLS) | No |
| Sequence | With the Actin-Chromobody plasmid you obtain the sequence of the Alpaca antibody to Actin fused to TagRFP or TagGFP2, as well as the full vector sequence. |
| Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
| Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy; super-resolution microscopy e.g. STED |
| Storage Condition | Shipped at ambient temperature. Store at -20 °C |
Documentation
| SDS |
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| acr_SDS_Actin-Chromobody® plasmid (TagRFP) (EN) |
| Datasheet |
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| acr_Datasheet_Actin-Chromobody® plasmid (TagRFP) |
| Brochure |
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| Chromotek nanobodies brochure (PDF) |
Publications
| Application | Title |
|---|---|
Nat Methods Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. | |
Nat Commun Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases. | |
Nat Commun tmem33 is essential for VEGF-mediated endothelial calcium oscillations and angiogenesis. | |
EMBO J F-actin patches associated with glutamatergic synapses control positioning of dendritic lysosomes. | |
Elife A mechanosensing mechanism controls plasma membrane shape homeostasis at the nanoscale |