Validation Data Gallery
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Tested Applications
| Positive WB detected in | Trichostatin A treated NIH/3T3 cells, Trichostatin A treated HSC-T6 cells |
| Positive IF/ICC detected in | Trichostatin A treated HeLa cells |
| Positive Dot Blot detected in | / / |
| Positive ChIP-qPCR detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| DOT BLOT | DOT BLOT : 1:10-1:100 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86768-1-RR targets Acetyl-Histone H4 (Lys91) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | histone cluster 1, H4a |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC069654 |
| Gene Symbol | HIST1H4A |
| Gene ID (NCBI) | 8359 |
| RRID | AB_3745109 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62805 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Acetyl-Histone H4 (Lys91) antibody 86768-1-RR | Download protocol |
| WB protocol for Acetyl-Histone H4 (Lys91) antibody 86768-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |