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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse testis tissue using Acetyl-Histone H3 (Lys23) Recombinant antibody (82838-2-RR)

    IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (HIST1H3A antibody) at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Acetyl-Histone H3 (Lys23) Recombinant antibody (82838-2-RR)

    IHC staining of mouse testis using 82838-2-RR (same clone as 82838-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using Acetyl-Histone H3 (Lys23) Recombinant antibody (82838-2-RR)

    IHC staining of rat testis using 82838-2-RR (same clone as 82838-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82838-2-RR (Acetyl-Histone H3 (Lys23) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H3 (Lys23) Recombinant antibody (82838-2-RR)

    IF Staining of HeLa using 82838-2-RR (same clone as 82838-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H3 (Lys23) antibody (82838-2-RR, Clone: 241137E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

  • Dot Blot experiment of peptide using Acetyl-Histone H3 (Lys23) Recombinant antibody (82838-2-RR)

    Dot Blot experiment of peptide using 82838-2-RR (same clone as 82838-2-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H3 (Lys23) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

  • ChIP experiment of HeLa cells using Acetyl-Histone H3 (Lys23) Recombinant antibody (82838-2-RR)

    ChIP experiment of HeLa using 82838-2-RR (same clone as 82838-2-PBS)

    Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H3 (Lys23) (82838-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82838-2-PBS in a different storage buffer formulation.

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Acetyl-Histone H3 (Lys23) Recombinant antibody, PBS Only

Acetyl-Histone H3 (Lys23) Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ChIP, Dot Blot, Indirect ELISA
Cat No. 82838-2-PBS
Clone No.241137E4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ChIP, Dot Blot, Indirect ELISA

Synonyms

Acetyl Histone, H3 Ac Lys23, H3 Acetyl K23, H3 Acetyl Lys23, H3 Acetyl Lysine23

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82838-2-PBS targets Acetyl-Histone H3 (Lys23) in IHC, IF/ICC, ChIP, Dot Blot, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name histone cluster 1, H3a
GenBank accession numberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.