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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using Acetyl-Histone H2B (Lys20) Recombinant antibody (84549-1-RR)

    WB analysis of NIH/3T3 using 84549-1-RR

    Trichostatin A treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84549-1-RR (Acetyl-Histone H2B (Lys20) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2B (Lys20) Recombinant antibody (84549-1-RR)

    IF Staining of HeLa using 84549-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using Acetyl-Histone H2B (Lys20) Recombinant antibody (84549-1-RR)

    IF Staining of NIH/3T3 using 84549-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated NIH/3T3 cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Acetyl-Histone H2B (Lys20) Recombinant antibody (84549-1-RR)

    IF Staining of HeLa using 84549-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Trichostatin A treated HeLa cells using Acetyl-Histone H2B (Lys20) antibody (84549-1-RR, Clone: 241204H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Dot Blot experiment of peptide using Acetyl-Histone H2B (Lys20) Recombinant antibody (84549-1-RR)

    Dot Blot experiment of peptide using 84549-1-RR

    Dot blot analysis was used to confirm the specificity of 84549-1-RR Acetyl-Histone H2B (Lys20) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.

  • ChIP experiment of HeLa cells using Acetyl-Histone H2B (Lys20) Recombinant antibody (84549-1-RR)

    ChIP experiment of HeLa using 84549-1-RR

    Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 3 µg of Acetyl-Histone H2B (Lys20) (84549-1-RR) or 3 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

Acetyl-Histone H2B (Lys20) Recombinant monoclonal antibody

Acetyl-Histone H2B (Lys20) Uni-rAb® Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR
Cat No. 84549-1-RR
Clone No.241204H8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR

Synonyms

Ac Histone H2B, Ac Histone H2B (Lys20), Ac Histone H2B Lys20, Ac Histone H2B-Lys20, Acetyl Histone

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IF/ICC detected inHeLa cells, Trichostatin A treated HeLa cells, Trichostatin A treated NIH/3T3 cells
Positive Dot Blot detected inpeptide
Positive ChIP-qPCR detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
CHIP-QPCRCHIP-QPCR : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84549-1-RR targets Acetyl-Histone H2B (Lys20) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
Observed molecular weight15 kDa
GenBank accession numberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3672056
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ16778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for Acetyl-Histone H2B (Lys20) antibody 84549-1-RRDownload protocol
WB protocol for Acetyl-Histone H2B (Lys20) antibody 84549-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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