Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83171-7-RR (Acetyl-Histone H2B (Lys15) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 83171-7-PBS in a different storage buffer formulation.

Dot blot analysis was used to confirm the specificity of 83171-7-RR Acetyl-Histone H2B (Lys15) antibody. peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 83171-7-PBS in a different storage buffer formulation.

Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2B (Lys15) (83171-7-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83171-7-PBS in a different storage buffer formulation.