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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Ace2 Polyclonal antibody (32628-1-AP)

    WB analysis using 32628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32628-1-AP (Ace2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Ace2 Polyclonal antibody (32628-1-AP)

    IHC staining of mouse heart using 32628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 32628-1-AP (Ace2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using Ace2 Polyclonal antibody (32628-1-AP)

    IHC staining of mouse heart using 32628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 32628-1-AP (Ace2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Ace2 Polyclonal antibody

Ace2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 32628-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ACE-related carboxypeptidase, Angiotensin-converting enzyme 2, EC:3.4.17.-, EC:3.4.17.23, Processed angiotensin-converting enzyme 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, mouse small intestine tissue, rat small intestine tissue
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32628-1-AP targets Ace2 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Ace2 fusion protein Eg2834 相同性解析による交差性が予測される生物種
Full Name angiotensin I converting enzyme (peptidyl-dipeptidase A) 2
Calculated molecular weight92 kDa
Observed molecular weight110 kDa, 120 kDa
GenBank accession numberNM_001130513.1
Gene Symbol Ace2
Gene ID (NCBI) 70008
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ8R0I0-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACE2 (Angiotensin-Converting Enzyme 2) is a key regulator of the renin-angiotensin system (RAS) and plays a crucial role in maintaining cardiovascular homeostasis. It is also the functional receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus responsible for COVID-19.

Protocols

Product Specific Protocols
WB protocol for Ace2 antibody 32628-1-APDownload protocol
IHC protocol for Ace2 antibody 32628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols