Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells, HepG2 cells |
| Positive IHC detected in | mouse testis tissue, human placenta tissue, mouse stomach tissue, human testis tissue, rat stomach tissue, human stomach tissue, human pancreas tissue, rat liver tissue, mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
15042-1-AP targets AZI2/NAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6892 Product name: Recombinant human AZI2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC001139 Sequence: MDALVEDDICILNHEKAHKRDTVTPVSIYSGDESVASHFALVTAYEDIKKRLKDSEKENSLLKKRIRFLEEKLIARFEEETSSVGREQVNKAYHAYREVCIDRDNLKSKLDKMNKDNSESLKVLNEQLQSKEVELLQLRTEVETQQVMRNLNPPSSNWEVEKLSCDLKIHGLEQELELMRKECSDLKIELQKAKQTDPYQEDNLKSRDLQKLSISSDNMQHAYWELKREMSNLHLVTQVQAELLRKLKTSTAIKKGKSLFTGNH 相同性解析による交差性が予測される生物種 |
| Full Name | 5-azacytidine induced 2 |
| Calculated molecular weight | 45 kDa |
| Observed molecular weight | 45 kDa |
| GenBank accession number | BC001139 |
| Gene Symbol | AZI2 |
| Gene ID (NCBI) | 64343 |
| RRID | AB_2878103 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6S1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AZI2, also known as NAP1, is a TNF receptor (TNFR)-associated factor family member-associated NF-κB activator-binding kinase 1-binding protein that regulates the production of IFNs. It is an adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
| IHC protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
| IP protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
| WB protocol for AZI2/NAP1 antibody 15042-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res FIP200 suppresses immune checkpoint therapy responses in breast cancers by limiting AZI2/TBK1/IRF signaling independent of its canonical autophagy function. | ||
Autophagy AZI2 mediates TBK1 activation at unresolved selective autophagy cargo receptor complexes with implications for CD8 T-cell infiltration in breast cancer | ||
EMBO J Phosphorylation tunes p62 condensates to drive autophagic degradation of ubiquitinated proteins. | ||
Cell Rep Phospho-proteome profiling in human neurons reveals targets of TBK1 in ALS/FTD-associated autophagy networks. | ||
Front Immunol Increased IKKϵ protein stability ensures efficient type I interferon responses in conditions of TBK1 deficiency | ||
J Cell Biol NAK-associated protein 1/NAP1 activates TBK1 to ensure accurate mitosis and cytokinesis |