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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AZI1 Recombinant monoclonal antibody (86809-3-RR)

    WB analysis using 86809-3-RR (same clone as 86809-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86809-3-RR (AZI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86809-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using AZI1 Recombinant monoclonal antibody (86809-3-RR)

    WB analysis using 86809-3-RR (same clone as 86809-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86809-3-RR (AZI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86809-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AZI1 Recombinant monoclonal antibody (86809-3-RR)

    IF Staining of HeLa using 86809-3-RR (same clone as 86809-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AZI1 antibody (86809-3-RR, Clone: 251839D3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86809-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86809-3-RR

    Affinity and Kinetic Characterization of 86809-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86809-3-RR against Human AZI1 were performed. The affinity constant is 0.704 nM.

AZI1 Recombinant monoclonal antibody, PBS Only

AZI1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86809-3-PBS
Clone No.251839D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

5 azacytidine induced 1, 5-azacytidine-induced protein 1, AZ1, CEP131, KIAA1118

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86809-3-PBS targets AZI1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag22699

Product name: Recombinant human AZI1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 730-1080 aa of BC136578

Sequence: HEAELLQSDERASQRCLRQAEELREQLEREKEALGQQERERARQRFQQHLEQEQWALQQQRQRLYSEVAEERERLGQQAARQRAELEELRQQLEESSSALTRALRAEFEKGREEQERRHQMELNTLKQQLELERQAWEAGRTRKEEAWLLNREQELREEIRKGRDKEIELVIHRLEADMALAKEESEKAAESRIKRLRDKYEAELSELEQSERKLQERCSELKGQLGEAEGENLRLQGLVRQKERALEDAQAVNEQLSSERSNLAQVIRQEFEDRLAASEEETRQAKAELATLQARQQLELEEVHRRVKTALARKEEAVSSLRTQHEAAVKRADHLEELLEQHRRPTPSTK

相同性解析による交差性が予測される生物種
Full Name 5-azacytidine induced 1
Calculated molecular weight 1083 aa, 122 kDa
Observed molecular weight117-130 kDa
GenBank accession numberBC136578
Gene Symbol AZI1
Gene ID (NCBI) 22994
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9UPN4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

AZI1, also named as CEP131 and KIAA1118, may play a role in spermatogenesis. It is located at the ciliary base and required for cilium formation.

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