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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Multi-rAb® AXL Multi-Recombinant antibody (RMX00064)

    WB analysis using RMX00064

    Various lysates were subjected to SDS PAGE followed by western blot with RMX00064 (AXL antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Multi-rAb® AXL Multi-Recombinant antibody (RMX00064)

    IF Staining of HeLa using RMX00064

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AXL antibody (RMX00064) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Multi-rAb® AXL Multi-Recombinant antibody

AXL Multi-Recombinant Antibody for WB, ELISA
Cat No. RMX00064

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.7.10.1, Tyrosine-protein kinase receptor UFO, UFO

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:14000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

RMX00064 targets AXL in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Multi-Recombinant
Type Antibody
Immunogen

Recombinant protein

相同性解析による交差性が予測される生物種
Full Name AXL receptor tyrosine kinase
Calculated molecular weight98kDa
Observed molecular weight130-140 kDa
GenBank accession numberNM_021913.4
Gene Symbol AXL
Gene ID (NCBI) 558
RRIDAB_3750114
Conjugate Unconjugated
Form
FormLiquid
Purification MethodN/A
UNIPROT IDP30530-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

Protocols

Product Specific Protocols
WB protocol for AXL antibody RMX00064Download protocol
Standard Protocols
Click here to view our Standard Protocols
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