Validation Data Gallery
Tested Applications
| Positive WB detected in | DU 145 cells, HeLa cells, HepG2 cells, human liver tissue |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 38 publications below |
| IHC | See 10 publications below |
| IF | See 9 publications below |
| IP | See 3 publications below |
| ELISA | See 1 publications below |
| CoIP | See 3 publications below |
Product Information
13196-1-AP targets AXL in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3898 Product name: Recombinant human AXL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 545-894 aa of BC032229 Sequence: GEFGAVMEGQLNQDDSILKVAVKTMKIAICTRSELEDFLSEAVCMKEFDHPNVMRLIGVCFQGSERESFPAPVVILPFMKHGDLHSFLLYSRLGDQPVYLPTQMLVKFMADIASGMEYLSTKRFIHRDLAARNCMLNENMSVCVADFGLSKKIYNGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWSFGVTMWEIATRGQTPYPGVENSEIYDYLRRGNRLKQPADCLDGLYALMSRCWELNPQDRPSFTELREDLENTLKALPPAQEPDEILYVNMDEGGGYPEPPGAAGGADPPTQPDPKDSCSCLTAAEVHPAGRYVLCPSTTPSPAQPADRGSPAAPGQEDGA 相同性解析による交差性が予測される生物種 |
| Full Name | AXL receptor tyrosine kinase |
| Calculated molecular weight | 894 aa, 98 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC032229 |
| Gene Symbol | AXL |
| Gene ID (NCBI) | 558 |
| RRID | AB_10642006 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30530 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for AXL antibody 13196-1-AP | Download protocol |
| WB protocol for AXL antibody 13196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
ACS Nano Personalized Pathogenic Nanoplastic Coronas Orchestrate Efferocytosis-Driven Immune Evasion in Lung Adenocarcinoma. | ||
Theranostics CD146(+) pericyte-like lung cancer brain metastatic stem cells promote tumor angiogenesis through dual regulatory effects on the VEGF/VEGFR axis. | ||
Adv Sci (Weinh) EGFR Targeted Liposomal PROTAC Assisted With Epigenetic Regulation as an Efficient Strategy for Osimertinib-Resistant Lung Cancer Therapy | ||
Cell Death Dis Identification of PRRG1 as a possible molecular target of pancreatic cancer. | ||
Cell Death Dis Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop
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