Validation Data Gallery
Tested Applications
| Positive WB detected in | 769-P cells, 786-O cells, Caki-1 cells, Caki-2 cells, OS-RC-2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27691-1-AP targets AVPR2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24835 Product name: Recombinant human AVPR2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-83 aa of BC112181 Sequence: MLMASTTSAVPGHPSLPSLPSNSSQERPLDTRDPLLARAELALLSIVFVAVALSNGLVLAALARRGRRGHWAPIHVFIGHLCL 相同性解析による交差性が予測される生物種 |
| Full Name | arginine vasopressin receptor 2 |
| Calculated molecular weight | 371 aa, 40 kDa |
| Observed molecular weight | 49 kDa |
| GenBank accession number | BC112181 |
| Gene Symbol | AVPR2 |
| Gene ID (NCBI) | 554 |
| RRID | AB_3742249 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P30518 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AVPR2 is a Gs-coupled vasopressin receptor essential for renal water reabsorption, acting through cAMP-PKA-dependent trafficking of aquaporin-2. Localized to principal cells of the collecting duct, AVPR2 integrates hormonal cues to maintain osmotic and fluid homeostasis. Mutations lead to X-linked nephrogenic diabetes insipidus, while rare activating variants cause SIADH. Because of its central role in renal physiology and clear clinical associations, AVPR2 is a key target in water-balance disorders and pharmacologic modulation of diuresis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AVPR2 antibody 27691-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |