Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27758-1-AP targets AVL9 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26208 Product name: Recombinant human AVL9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 188-463 aa of BC027724 Sequence: RHKVLILFKLILLEKKVLFYISPVNKLVGALMTVLSLFPGMIEHGLSDCSQYRPRKSMSEDGGLQESNPCADDFVSASTADVSHTNLGTIRKVMAGNHGEDAAMKTEEPLFQVEDSSKGQEPNDTNQYLKPPSRPSPDSSESDWETLDPSVLEDPNLKEREQLGSDQTNLFPKDSVPSESLPITVQPQANTGQVVLIPGLISGLEEDQYGMPLAIFTKCTQDVLPQANHSFKFLITDRRITPRPLRRRLVSATLTNMLQEKSCWKKHIKCRRRVQK 相同性解析による交差性が予測される生物種 |
| Full Name | AVL9 homolog (S. cerevisiase) |
| Observed molecular weight | 72 kDa |
| GenBank accession number | BC027724 |
| Gene Symbol | AVL9 |
| Gene ID (NCBI) | 23080 |
| RRID | AB_3742254 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NBF6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AVL9 is primarily involved in cell migration and late secretory pathway transport . In yeast, Avl9p functions in post-Golgi vesicle-mediated transport, where loss of the protein causes defects in secretory vesicle generation and polarized growth . In humans, the protein has been shown to function in cell migration, though the precise molecular mechanism remains under investigation.(PMID: 34991461,PMID: 41542567)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AVL9 antibody 27758-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |