Validation Data Gallery
Tested Applications
| Positive WB detected in | SK-N-SH cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65031-1-Ig targets AURKB in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Recombinant Protein 相同性解析による交差性が予測される生物種 |
| Full Name | aurora kinase B |
| Calculated molecular weight | 39.2 kDa |
| GenBank accession number | BC013300 |
| Gene Symbol | AURKB |
| Gene ID (NCBI) | 9212 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96GD4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at 2-8°C. |
Background Information
Aurora kinase B(AURKB) is also named as AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5 and belongs to the Aurora subfamily. Aurora kinase B is a chromosomal passenger serine/threonine protein kinase that regulates accurate chromosomal segregation, cytokinesis, protein localization to the centromere and kinetochore, correct microtubule-kinetochore attachments, and regulation of the mitotic checkpoint(PMID:18311747). Expression levels of AURKB are increased in the anaplastic thyroid carcinoma cell lines ARO, Cal 62, and KAT-4(PMID:15562011).