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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AURKAIP1 Polyclonal antibody (17528-1-AP)

    IF Staining of HepG2 using 17528-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using AURKAIP1 antibody (17528-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

AURKAIP1 Polyclonal antibody

AURKAIP1 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 17528-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

28S ribosomal protein S38, mitochondrial, AIP, AKIP, AURKA interacting protein, AURKA-interacting protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17528-1-AP targets AURKAIP1 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11444

Product name: Recombinant human AURKAIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-199 aa of BC062333

Sequence: MLLGRLTSQLLRAVPWAGGRPPWPVSGVLGSRVCGPLYSTSPAGPGRAASLPRKGAQLELEEMLVPRKMSVSPLESWLTARCFLPRLDTGTAGTVAPPQSYQCPPSQIGEGAEQGDEGVADAPQIQCKNVLKIRRRKMNHHKYRKLVKKTRFLRRKVQEGRLRRKQIKFEKDLRRIWLKAGLKEAPEGWQTPKIYLRGK

相同性解析による交差性が予測される生物種
Full Name aurora kinase A interacting protein 1
Calculated molecular weight 199 aa, 22 kDa
GenBank accession numberBC062333
Gene Symbol AURKAIP1
Gene ID (NCBI) 54998
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWT8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AURKAIP1 (Aurora-A kinase interacting protein 1) has been identified as an Aurora-A kinase interacting protein involving in two degradation pathways of Aurora-A, including proteasome-dependent pathway and Ub-independent pathway . Ectopic expression of AURKAIP1 resulted in the down-regulation of Aurora-A protein levels and thus, AURKAIP1 could be regarded as a cancer suppressor involving Aurora-A pathway.(PMID: 38040691,PMID: 17125467)

Protocols

Product Specific Protocols
IF protocol for AURKAIP1 antibody 17528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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