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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AURKA Recombinant antibody

AURKA Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230087H3

Cat no : 82906-1-RR

Synonyms

AIK, ARK 1, ARK1, AURA, AURKA, aurora kinase A, Aurora related kinase 1, Aurora/IPL1 related kinase 1, AURORA2, Breast tumor amplified kinase, BTAK, hARK1, STK15, STK6, STK7

Validation Data Gallery

Applications:
  • WB analysis using 82906-1-RR

    WB analysis using 82906-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82906-1-RR (AURKA antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy AURKA Ready-To-Use IHC Kit. AURKA primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

82906-1-RR targets AURKA in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen AURKA fusion protein Ag0362 相同性解析による交差性が予測される生物種
Full Name aurora kinase A
Calculated molecular weight 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC002499
Gene symbol AURKA
Gene ID (NCBI) 6790
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

AURKA(Aurora kinase A), also named as STK6, STK15 or AIK, belongs to the Ser/Thr protein kinase family. This protein may play a role in cell cycle regulation during anaphase and/or telophase by being involved in microtubule formation and/or stabilization. Some recent evidence revealed that AURKA may also be implicated in tumor development and progression. AURKA is expressed highly in testis and various proliferating cells, migrating as a 46 kDa protein in SDS PAGE.Nuclear staining for AURKA is weak or nonexistent in normal tissue but strong in tumor tissue(PMID:19107951).

Protocols

Product Specific Protocols
WB protocol for AURKA antibody 82906-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols