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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AUH Recombinant antibody (85421-1-RR)

    WB analysis using 85421-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85421-1-RR (AUH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AUH Recombinant monoclonal antibody (85421-1-RR)

    IHC staining of mouse brain using 85421-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85421-1-RR (AUH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85421-1-RR

    Affinity and Kinetic Characterization of 85421-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85421-1-RR against Human AUH were performed. The affinity constant is 29.3 nM.

AUH Recombinant monoclonal antibody

AUH Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 85421-1-RR
Clone No.242854F12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

242854F12, 3-MG-CoA hydratase, AU-binding protein/enoyl-CoA hydratase, AU-specific RNA-binding enoyl-CoA hydratase, EC:4.2.1.18

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue, fetal human brain tissue, human kidney tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85421-1-RR targets AUH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11265

Product name: Recombinant human AUH protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-310 aa of BC020722

Sequence: MAAAVAAAPGALGSLHAGGARLVAACSAWLCPGLRLPGSLAGRRAGPAIWAQGWVPAAGGPAPKRGYSSEMKTEDELRVRHLEEENRGIVVLGINRAYGKNSLSKNLIKMLSKAVDALKSDKKVRTIIIRSEVPGIFCAANLPVPTIAAIDGLALGGGLELALACDIRVAASSAKMGLVETKLAIIPGGGGTQRLPRAIGMSLAKELIFSARVLDGKEAKAVGLISHVLEQNQEGDAAYRKALDLAREFLPQGPVAMRVAKLAINQGMEVDLVTGLAIEEACYAQTIPTKDRLEGLLAFKEKRPPRYKGE

相同性解析による交差性が予測される生物種
Full Name AU RNA binding protein/enoyl-Coenzyme A hydratase
Calculated molecular weight 310 aa, 33 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC020722
Gene Symbol AUH
Gene ID (NCBI) 549
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ13825
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AUH antibody 85421-1-RRDownload protocol
IHC protocol for AUH antibody 85421-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols