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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATRX Polyclonal antibody (20495-1-AP)

    WB analysis using 20495-1-AP (same clone as 20495-1-PBS)

    A549 cells were subjected to Tris-Acetate gel system followed by western blot with 20495-1-AP (ATRX antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATRX Polyclonal antibody (20495-1-AP)

    IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using ATRX Polyclonal antibody (20495-1-AP)

    IHC staining of rat brain using 20495-1-AP (same clone as 20495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20495-1-AP (ATRX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATRX Polyclonal antibody (20495-1-AP)

    IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ATRX Polyclonal antibody (20495-1-AP)

    IF Staining of A549 using 20495-1-AP (same clone as 20495-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using ATRX antibody (20495-1-AP) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 20495-1-PBS in a different storage buffer formulation.

ATRX Polyclonal antibody, PBS Only

ATRX Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20495-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20495-1-PBS targets ATRX in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13984

Product name: Recombinant human ATRX protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-87 aa of BC002521

Sequence: MTAEPMSESKLNTLVQKLHDFLAHSSEESEETSSPPRLAMNQNTDKISGSGSNSDMMENSKEEGTSSSEKSKSSGSSRSKRKPSIVN

相同性解析による交差性が予測される生物種
Full Name alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog, S. cerevisiae)
Calculated molecular weight 2492 aa, 283 kDa
Observed molecular weight280-300 kDa
GenBank accession numberBC002521
Gene Symbol ATRX
Gene ID (NCBI) 546
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP46100
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The α-thalassemia mental retardation X-linked protein (ATRX) is a member of the Switch 2, sucrose non-fermenting 2 (SWI2/SNF2) family of helicases/ATPases that exhibits chromatin remodeling activity. ATRX contains an atypical plant homeodomain (PHD) finger domain that recognizes a combinatorial modification pattern on histone H3 tails. ATRX mutations in cancer are most commonly found in pediatric and adolescent malignancies including glioma, neuroblastoma, adrenocortical carcinoma and osteosarcoma(PMID: 32015155).

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