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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ATRAID Polyclonal antibody (25548-1-AP)

    WB analysis of mouse brain using 25548-1-AP (same clone as 25548-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25548-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using ATRAID Polyclonal antibody (25548-1-AP)

    WB analysis of mouse kidney using 25548-1-AP (same clone as 25548-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25548-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ATRAID Polyclonal antibody (25548-1-AP)

    WB analysis of HEK-293 using 25548-1-AP (same clone as 25548-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 25548-1-AP (ATRAID antibody at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 25548-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25548-1-PBS targets ATRAID in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATRAID fusion protein Ag22168 相同性解析による交差性が予測される生物種
Full Name chromosome 2 open reading frame 28
Calculated molecular weight 229 aa, 25 kDa
Observed molecular weight 28-35 kDa
GenBank accession numberBC035850
Gene Symbol ATRAID
Gene ID (NCBI) 51374
RRIDAB_2880128
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UW56
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATRAID, also named as APR3, p18, C2orf28, promotes osteoblast cell differentiation and terminal mineralization. It plays a role in inducing the cell cycle arrest via inhibiting CCND1 expression in all-trans-retinoic acid (ATRA) signal pathway. This antibody recognizes all the three isoforms (20-24 kDa, 16-19 kDa and 28-30 kDa).