Validation Data Gallery
Tested Applications
| Positive WB detected in | pig skeletal muscle tissue, human skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88342-1-RR targets CARNS1 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag22761 Product name: Recombinant human ATPGD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 443-827 aa of BC036557 Sequence: MTEHRRDEENARLLAELVRARGLKLDGCFSYWDDCLVLTALLCQELGLPCSSPAALRLAKQKSLTQLHLLHHHGPPWPAPSLHAVPCCPLESEADVERAVHQVPLPGVMKLEFGAGAVGVRLVEDAPQCHEHFSRITRDLQGEADHPGIGLGWGNAMLLMEFVEGTEHDVDLVLFGGRLLAAFVSDNGPTRLPGFTETAACMPTGLAPEQEAQMVQAAFRCCLGCGLLDGVFNVELKLTGAGPRLIEINPRMGGFYLRDWILELYGVDLLLAAVMVACGLRPALPTRPRARGHLVGVMCLVSQHLQALSSTASRETLQALHDRGLLRLNLLEEALVPGEYEEPYCSVACAGPSPTEARLRLLGLCQGLGIDGPSYPVAHFLSHFK 相同性解析による交差性が予測される生物種 |
| Full Name | ATP-grasp domain containing 1 |
| Calculated molecular weight | 827 aa, 88 kDa |
| Observed molecular weight | 88-103 kDa |
| GenBank accession number | BC036557 |
| Gene Symbol | CARNS1 |
| Gene ID (NCBI) | 57571 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | A5YM72 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CARNS1, located at human chromosome 11q13.2, encodes carnosine synthase 1 belonging to the ATP-grasp ATPase family (EC 6.3.2.11). It mediates ATP-dependent synthesis of carnosine in skeletal muscle and homocarnosine in the central nervous system, two vital histidine-containing dipeptides with antioxidant and pH-buffering effects. This gene is highly conserved across vertebrates and preferentially expressed in brain and muscle tissues. Aberrant CARNS1 expression disturbs dipeptide homeostasis, linking to aging-related sensory decline and tumor progression via impaired carnosine-mediated tumor suppression. (PMID: 20097752)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CARNS1 antibody 88342-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |