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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using ATPBD4 Polyclonal antibody (23993-1-AP)

    WB analysis of NIH/3T3 using 23993-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23993-1-AP (ATPBD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using ATPBD4 Polyclonal antibody (23993-1-AP)

    WB analysis of L02 using 23993-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 23993-1-AP (ATPBD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using ATPBD4 Polyclonal antibody (23993-1-AP)

    IF Staining of NIH/3T3 using 23993-1-AP

    Immunofluorescent analysis of NIH/3T3 cells using 23993-1-AP (ATPBD4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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ATPBD4 Polyclonal antibody

ATPBD4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23993-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

ATP binding domain 4, ATPBD4, Diphthamide synthase, Diphthamide synthetase, Diphthine-ammonia ligase, DPH6, Protein DPH6 homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, L02 cells
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

23993-1-AP targets ATPBD4 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATPBD4 fusion protein Ag21178 相同性解析による交差性が予測される生物種
Full Name ATP binding domain 4
Calculated molecular weight 267 aa, 30 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC008485
Gene Symbol ATPBD4
Gene ID (NCBI) 89978
RRIDAB_2879392
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L8W6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Diphthamide is a highly modified histidine in the transcription factor EEF2 that is conserved from yeast to human. ATPBD4, also known as DPH6, belongs to the Diphthine--ammonia ligase family. DPH6 amidase may catalyze the last step of diphthamide biosynthesis using ammonium and ATP to play a role in catalyzing the final step of diphthamide biosynthesis, amidation of diphthine to diphthamide (PubMed:23169644).

Protocols

Product Specific Protocols
WB protocol for ATPBD4 antibody 23993-1-APDownload protocol
IF protocol for ATPBD4 antibody 23993-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Brain Sci

Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells.

Authors - Tsung-Ming Hu
View Article