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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATPBD3 Recombinant monoclonal antibody (84318-4-RR)

    WB analysis using 84318-4-RR (same clone as 84318-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84318-4-RR (ATPBD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATPBD3 Recombinant monoclonal antibody (84318-4-RR)

    IHC staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATPBD3 Recombinant monoclonal antibody (84318-4-RR)

    IHC staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84318-4-RR (ATPBD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse kidney tissue using ATPBD3 Recombinant monoclonal antibody (84318-4-RR)

    IF Staining of mouse kidney using 84318-4-RR (same clone as 84318-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using ATPBD3 antibody (84318-4-RR, Clone: 241630C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84318-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84318-4-RR

    Affinity and Kinetic Characterization of 84318-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84318-4-RR against Human ATPBD3 were performed. The affinity constant is 0.406 nM.

ATPBD3 Recombinant monoclonal antibody, PBS Only

ATPBD3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 84318-4-PBS
Clone No.241630C4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

241630C4, CTU1, NCS6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84318-4-PBS targets ATPBD3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag35037

Product name: Recombinant human ATPBD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 50-200 aa of NM_145232

Sequence: PGAVVAVGASGGKDSTVLAHVLRALAPRLGISLQLVAVDEGIGGYRDAALAAVRRQAARWELPLTVVAYEDLFGGWTMDAVARSTAGSGRSRSCCTFCGVLRRRALEEGARRVGATHIVTGHNADDMAETVLMNFLRGDAGRLARGGGLGS

相同性解析による交差性が予測される生物種
Full Name ATP binding domain 3
Calculated molecular weight36 kDa
Observed molecular weight 35 kDa
GenBank accession numberNM_145232
Gene Symbol ATPBD3
Gene ID (NCBI) 90353
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ7Z7A3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATPBD3, also known as CTU1, NCS6, is a subunit of a highly conserved protein complex involved in the thiolation of wobble U34(PMID: 18391219). ATPBD3 directly binds tRNAs and probably acts by catalyzing the adenylation of tRNAs, an intermediate required for 2-thiolation.

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