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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATPB Monoclonal antibody (66600-1-Ig)

    WB analysis using 66600-1-Ig (same clone as 66600-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66600-1-Ig (ATPB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ATPB Monoclonal antibody (66600-1-Ig)

    IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ATPB Monoclonal antibody (66600-1-Ig)

    IHC staining of human liver using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ATPB Monoclonal antibody (66600-1-Ig)

    IHC staining of mouse skeletal muscle using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using ATPB Monoclonal antibody (66600-1-Ig)

    IHC staining of mouse skeletal muscle using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66600-1-Ig (ATPB antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ATPB Monoclonal antibody (66600-1-Ig)

    IF Staining of U2OS using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATPB Monoclonal antibody (66600-1-Ig)

    IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ATPB Monoclonal antibody (66600-1-Ig)

    IF Staining of HepG2 using 66600-1-Ig (same clone as 66600-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATPB antibody (66600-1-Ig, Clone: 1A11B9 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66600-1-PBS in a different storage buffer formulation.

ATPB Monoclonal antibody, PBS Only

ATPB Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66600-1-PBS
Clone No.1A11B9

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

1A11B9, ATP synthase F1 subunit beta, ATP5B, ATP5F1B, ATPMB

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66600-1-PBS targets ATPB in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag11177

Product name: Recombinant human ATPB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 181-529 aa of BC016512

Sequence: EILVTGIKVVDLLAPYAKGGKIGLFGGAGVGKTVLIMELINNVAKAHGGYSVFAGVGERTREGNDLYHEMIESGVINLKDATSKVALVYGQMNEPPGARARVALTGLTVAEYFRDQEGQDVLLFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGTMQERITTTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAIAELGIYPAVDPLDSTSRIMDPNIVGSEHYDVARGVQKILQDYKSLQDIIAILGMDELSEEDKLTVSRARKIQRFLSQPFQVAEVFTGHMGKLVPLKETIKGFQQILAGEYDHLPEQAFYMVGPIEEAVAKADKLAEEHSS

相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide
Calculated molecular weight 529 aa, 57 kDa
Observed molecular weight45-57 kDa
GenBank accession numberBC016512
Gene Symbol ATPB
Gene ID (NCBI) 506
RRIDAB_2881960
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP06576
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATP5B, also named as ATPMB and ATPSB, belongs to the ATPase alpha/beta chains family. It is a high affinity HDL receptor for apolipoprotein A1. ATP5B is a subunit of mitochondrial ATP synthase (F1F0 ATP synthase or Complex V ). ATP5B has a calculated molecular mass of 57 kDa and ~5 kDa transit peptide can be removed in the mature form.

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