Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | human ovary cancer tissue, mouse ovary tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 15 publications below |
| IF | See 3 publications below |
Product Information
21565-1-AP targets ATP8A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16071 Product name: Recombinant human ATP8A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 430-527 aa of BC109317 Sequence: QNSQFGDEKTFSDSSLLENLQNNHPTAPIICEFLTMMAVCHTAVPEREGDKIIYQAASPDEGALVRAAKQLNFVFTGRTPDSVIIDSLGQEERYELLN 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1 |
| Calculated molecular weight | 1164 aa, 131 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC109317 |
| Gene Symbol | ATP8A1 |
| Gene ID (NCBI) | 10396 |
| RRID | AB_10734587 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2Q0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ATP8A1 antibody 21565-1-AP | Download protocol |
| WB protocol for ATP8A1 antibody 21565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Neurosci Adaptor protein AP-3 produces synaptic vesicles that release at high frequency by recruiting phospholipid flippase ATP8A1
| ||
Nat Commun Synaptic vesicle-omics in mice captures signatures of aging and synucleinopathy | ||
Cell Death Dis Loss of Atp8a2 drives neurodegeneration through the dysregulation of spatiotemporal phosphatidylserine externalization in mature neurons. | ||
Cell Death Discov 2-Aminoethoxydiphenylborate (2-APB) inhibits release of phosphatidylserine-exposing extracellular vesicles from platelets. | ||
Proc Natl Acad Sci U S A AP-3-dependent targeting of flippase ATP8A1 to lamellar bodies suppresses activation of YAP in alveolar epithelial type 2 cells. | ||
EMBO J Transport through recycling endosomes requires EHD1 recruitment by a phosphatidylserine translocase. |