Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85288-2-RR targets ATP7B-Specific in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, Cu++ transporting, beta polypeptide |
| Calculated molecular weight | 157 kDa |
| Observed molecular weight | 150 kDa |
| GenBank accession number | NM_000053 |
| Gene Symbol | ATP7B |
| Gene ID (NCBI) | 540 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35670 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP7B, also named as PWD, WC1 and WND, belongs to the cation transport ATPase (P-type) family and Type IB subfamily. ATP7B is involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. ATP7B catalyzes the reaction: ATP + H2O + Cu2+(In) = ADP + phosphate + Cu2+(Out). Defects in ATP7B are the cause of Wilson disease (WD). This antibody is specific to ATP7B.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP7B-Specific antibody 85288-2-RR | Download protocol |
| IHC protocol for ATP7B-Specific antibody 85288-2-RR | Download protocol |
| IF protocol for ATP7B-Specific antibody 85288-2-RR | Download protocol |
| FC protocol for ATP7B-Specific antibody 85288-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |