ATP6V1G2 Polyclonal antibody
ATP6V1G2 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC,ELISA
Conjugate
Unconjugated
Cat no : 25316-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 3 publications below |
Product Information
The immunogen of 25316-1-AP is ATP6V1G2 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | ATP6V1G2 fusion protein Ag18007 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G2 |
| Calculated molecular weight | 118 aa, 14 kDa |
| Observed molecular weight | 14 kDa |
| GenBank accession number | BC119726 |
| Gene symbol | ATP6V1G2 |
| Gene ID (NCBI) | 534 |
| RRID | AB_2880027 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6V1G2 antibody 25316-1-AP | Download protocol |
| IHC protocol for ATP6V1G2 antibody 25316-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Microbiol The interferon-inducible isoform of NCOA7 inhibits endosome-mediated viral entry. | ||
EBioMedicine A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes. | ||
EBioMedicine Specific V-ATPase expression sub-classifies IDHwt lower-grade gliomas and impacts glioma growth in vivo. |
