Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse kidney using 16274-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 16274-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC staining of human lung using 16274-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human heart using 16274-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 16274-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 16274-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 16274-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

ATP6V1C2 Polyclonal antibody

ATP6V1C2 Polyclonal Antibody for WB, ELISA

Cat No. 16274-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

ATP6C2, V-ATPase subunit C 2, VMA5, V-type proton ATPase subunit C 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

mouse kidney tissue, human kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

16274-1-AP targets ATP6V1C2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9349 Product name: Recombinant human ATP6V1C2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-381 aa of BC012142 Sequence: MSEFWLISAPGDKENLQALERMNTVTSKSNLSYNTKFAIPDFKVGTLDSLVGLSDELGKLDTFAESLIRRMAQSVVEVMEDSKGKVQEHLLANGVDLTSFVTHFEWDMAKYPVKQPLVSVVDTIAKQLAQIEMDLKSRTAAYDTLKTNLENLEKKSMGNLFTRTLSDIVSKEDFVLDSEYLVTLLVIVPKPNYSQWQKTYESLSDMVVPRSTKLITEDKEGGLFTVTLFRKVIEDFKTKAKENKFTVREFYYDEKEIEREREEMARLLSDKKQQYGPLLRWLKVNFSEAFIAWIHIKALRVFVESVLRYGLPVNFQAVLLQPHKKSSTKRLREVLNSVFRHLDEVAATSILDASVEIPGLQLNNQDYFPYVYFHIDLSLLD 相同性解析による交差性が予測される生物種
Full Name	ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2
Calculated molecular weight	381 aa, 44 kDa
Observed molecular weight	44 kDa
GenBank accession number	BC012142
Gene Symbol	ATP6V1C2
Gene ID (NCBI)	245973
RRID	AB_2062522
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NEY4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for ATP6V1C2 antibody 16274-1-AP	Download protocol
WB protocol for ATP6V1C2 antibody 16274-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Environ Health Perspect Effects of Inorganic Arsenic on Human Prostate Stem-Progenitor Cell Transformation, Autophagic Flux Blockade, and NRF2 Pathway Activation. Authors - Lishi Xie KD Validated View Article
	WB	Nat Commun Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis Authors - Tze Mun Loo View Article
human	WB,IHC	Front Genet High Expression of ATP6V1C2 Predicts Unfavorable Overall Survival in Patients With Colon Adenocarcinoma Authors - Guanghua Li View Article
human	WB	Pathol Res Pract Comprehensive analysis of ATP6V1s family member, ATP6V1C2, with prognostic and drug development values in colorectal cancer Authors - Jun Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 16274-1-AP

WB analysis of human kidney using 16274-1-AP

IHC staining of human lung using 16274-1-AP

IHC staining of human heart using 16274-1-AP

IHC staining of human kidney using 16274-1-AP

IHC staining of human placenta using 16274-1-AP

IHC staining of human liver using 16274-1-AP

ATP6V1C2 Polyclonal antibody

ATP6V1C2 Polyclonal Antibody for WB, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Effects of Inorganic Arsenic on Human Prostate Stem-Progenitor Cell Transformation, Autophagic Flux Blockade, and NRF2 Pathway Activation.

Senescence-associated lysosomal dysfunction impairs cystine deprivation-induced lipid peroxidation and ferroptosis

High Expression of ATP6V1C2 Predicts Unfavorable Overall Survival in Patients With Colon Adenocarcinoma

Comprehensive analysis of ATP6V1s family member, ATP6V1C2, with prognostic and drug development values in colorectal cancer

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