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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

ATP6V1C2 Polyclonal antibody

ATP6V1C2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16274-1-AP

Synonyms

ATP6C2, ATP6V1C2, V ATPase subunit C 2, VMA5

Validation Data Gallery

Applications:
  • WB analysis of mouse kidney using 16274-1-AP

    WB analysis of mouse kidney using 16274-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of human kidney using 16274-1-AP

    WB analysis of human kidney using 16274-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 16274-1-AP

    IHC staining of human testis using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 16274-1-AP

    IHC staining of human testis using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human liver using 16274-1-AP

    IHC staining of human liver using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16274-1-AP

    IHC staining of human liver using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung using 16274-1-AP

    IHC staining of human lung using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 16274-1-AP

    IHC staining of human lung using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 16274-1-AP

    IHC staining of human heart using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16274-1-AP

    IHC staining of human heart using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human kidney using 16274-1-AP

    IHC staining of human kidney using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 16274-1-AP

    IHC staining of human kidney using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human placenta using 16274-1-AP

    IHC staining of human placenta using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 16274-1-AP

    IHC staining of human placenta using 16274-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16274-1-AP (ATP6V1C2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse kidney tissue, human kidney tissue
Positive IHC detected inhuman testis tissue, human heart tissue, human kidney tissue, human liver tissue, human lung tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16274-1-AP targets ATP6V1C2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP6V1C2 fusion protein Ag9349 相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2
Calculated molecular weight 381 aa, 44 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC012142
Gene symbol ATP6V1C2
Gene ID (NCBI) 245973
RRIDAB_2062522
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATP6V1C2 antibody 16274-1-APDownload protocol
IHC protocol for ATP6V1C2 antibody 16274-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Environ Health Perspect

Effects of Inorganic Arsenic on Human Prostate Stem-Progenitor Cell Transformation, Autophagic Flux Blockade, and NRF2 Pathway Activation.

Authors - Lishi Xie
  • KD Validated
View Article
humanWB,IHC

Front Genet

High Expression of ATP6V1C2 Predicts Unfavorable Overall Survival in Patients With Colon Adenocarcinoma

Authors - Guanghua Li
View Article