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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP6V1A Monoclonal antibody (68440-1-Ig)

    WB analysis using 68440-1-Ig (same clone as 68440-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68440-1-Ig (ATP6V1A antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ATP6V1A Monoclonal antibody (68440-1-Ig)

    WB analysis of HeLa using 68440-1-Ig (same clone as 68440-1-PBS)

    WB result of ATP6V1A antibody (68440-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP6V1A transfected HeLa cells. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1A Monoclonal antibody (68440-1-Ig)

    IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ATP6V1A Monoclonal antibody (68440-1-Ig)

    IHC staining of mouse kidney using 68440-1-Ig (same clone as 68440-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68440-1-Ig (ATP6V1A antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ATP6V1A Monoclonal antibody (68440-1-Ig)

    FC experiment of HeLa using 68440-1-Ig (same clone as 68440-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ATP6V1A Monoclonal antibody (68440-1-Ig, Clone:1C5G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 68440-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ATP6V1A Monoclonal antibody, PBS Only

ATP6V1A Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68440-1-PBS
Clone No.1C5G2

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, Indirect ELISA

Synonyms

1C5G2, ATP6A1, ATP6V1A1, EC:7.1.2.2, VA68

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68440-1-PBS targets ATP6V1A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10820

Product name: Recombinant human ATP6V1A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 268-617 aa of BC013138

Sequence: NSDVIIYVGCGERGNEMSEVLRDFPELTMEVDGKVESIMKRTALVANTSNMPVAAREASIYTGITLSEYFRDMGYHVSMMADSTSRWAEALREISGRLAEMPADSGYPAYLGARLASFYERAGRVKCLGNPEREGSVSIVGAVSPPGGDFSDPVTSATLGIVQVFWGLDKKLAQRKHFPSVNWLISYSKYMRALDEYYDKHFTEFVPLRTKAKEILQEEEDLAEIVQLVGKASLAETDKITLEVAKLIKDDFLQQNGYTPYDRFCPFYKTVGMLSNMIAFYDMARRAVETTAQSDNKITWSIIREHMGDILYKLSSMKFKDPLKDGEAKIKSDYAQLLEDMQNAFRSLED

相同性解析による交差性が予測される生物種
Full Name ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A
Calculated molecular weight 617 aa, 68 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC013138
Gene Symbol ATP6V1A
Gene ID (NCBI) 523
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP38606
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.

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