Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
33364-1-AP targets ATP6V0D2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38739 Product name: Recombinant human ATP6V0D2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 238-349 aa of BC065207 Sequence: ETLYPTFGKLYPEGLRLLAQAEDFDQMKNVADHYGVYKPLFEAVGGSGGKTLEDVFYEREVQMNVLAFNRQFHYGVFYAYVKLKEQEIRNIVWIAECISQRHRTKINSYIPI 相同性解析による交差性が予測される生物種 |
| Full Name | ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d2 |
| Calculated molecular weight | 40 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC065207 |
| Gene Symbol | ATP6V0D2 |
| Gene ID (NCBI) | 245972 |
| RRID | AB_3717331 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q8N8Y2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ATP6V0D2 is a subunit of the vacuolar proton-translocating ATPase (V-ATPase) complex, which is responsible for acidifying intracellular compartments and the extracellular environment. This protein is crucial for maintaining the acidic environment necessary for various cellular processes, including bone resorption by osteoclasts (PMID: 19113919). ATP6V0D2 is also involved in the regulation of tumor-associated macrophages, where it influences tumor progression through the lactate/ATP6V0D2/HIF-2α axis (PMID: 30431439).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATP6V0D2 antibody 33364-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |