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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP5S Polyclonal antibody (16335-1-AP)

    WB analysis using 16335-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16335-1-AP (ATP5S antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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ATP5S Polyclonal antibody

ATP5S Polyclonal Antibody for WB, ELISA
Cat No. 16335-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FB, DMAC2L, Distal membrane arm assembly complex 2-like protein, ATPW, ATP synthase-coupling factor B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, K-562 cells, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

16335-1-AP targets ATP5S in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP5S fusion protein Ag9486 相同性解析による交差性が予測される生物種
Full Name ATP synthase, H+ transporting, mitochondrial F0 complex, subunit s (factor B)
Calculated molecular weight127aa,15 kDa; 215aa,25 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC011549
Gene Symbol ATP5S
Gene ID (NCBI) 27109
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99766
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DMAC2L also known as ATP5S, belongs to the ATP synthase family, which is a group of enzymes that play a crucial role in cellular energy production. ATP synthase is the central enzyme in oxidative phosphorylation, responsible for the production of most of the ATP in mammalian organisms. ATP5S is the S subunit (also known as factor B) of mitochondrial FO complex of ATP synthase. It is an essential component of the H+ channel of the FO complex (PMID:7706317, 6143319).

Protocols

Product Specific Protocols
WB protocol for ATP5S antibody 16335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochem Genet

MiR-34a is Involved in the Decrease of ATP Contents Induced by Resistin Through Target on ATP5S in HepG2 Cells.

Authors - Fengyun Wen
View Article