Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 10910-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10910-1-AP
WB result of ATP5C1 antibody (10910-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP5C1 transfected HeLa cells.
WB analysis of mouse brain using 10910-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10910-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 10910-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 10910-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 10910-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10910-1-AP (ATP5C1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 10910-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10910-1-AP (ATP5C1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L); Phalloidin stains F-actin (Red).
IF Staining of HepG2 using 10910-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ATP5C1 antibody (10910-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of HepG2 using 10910-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10910-1-AP (ATP5C1 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

KD/KO Validated

ATP5C1 Polyclonal antibody

ATP5C1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10910-1-AP

Publications(15)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ATP synthase F1 subunit gamma, ATP5C, ATP5CL1, ATP5F1C, F-ATPase gamma subunit

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (15)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, human heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue
Positive IHC detected in	human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 13 publications below
IHC	See 1 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

10910-1-AP targets ATP5C1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1343 Product name: Recombinant human ATP5C1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC000931 Sequence: MFSRAGVAGLSAWTLQPQWIQVRNMATLKDITRRLKSIKNIQKITKSMKMVAAAKYARAERELKPARIYGLGSLALYEKADIKGPEDKKKHLLIGVSSDRGLCGAIHSSIAKQMKSEVATLTAAGKEVMLVGIGDKIRGILYRTHSDQFLVAFKEVGRKPPTFGDASVIALELLNSGYEFDEGSIIFNKFRSVISYKTEEKPIFSLNTVASADSMSIYDDIDADVLQNYQEYNLANIIYYSLKESTTSEQSARMTAMDNASKNASEMIDKLTLTFNRTRQAVITKELIEIISGAAALD 相同性解析による交差性が予測される生物種
Full Name	ATP synthase, H+ transporting, mitochondrial F1 complex, gamma polypeptide 1
Calculated molecular weight	33 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC000931
Gene Symbol	ATP5C1
Gene ID (NCBI)	509
RRID	AB_2877740
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P36542
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATP5C1(ATP synthase subunit gamma, mitochondrial) is also named as ATP5C, ATP5CL1 and belongs to the ATPase gamma chain family. Some scientists reported the complete sequence of the gene for the human ATP synthase gamma subunit and described tissue-specific isoforms of the subunit generated by alternative splicing of exon 9. The liver (L) isoform differed from the heart (H) isoform by the addition of a single amino acid (asp273) at the C terminus. (PMID:8227057).

Protocols

Product Specific Protocols
IF protocol for ATP5C1 antibody 10910-1-AP	Download protocol
IHC protocol for ATP5C1 antibody 10910-1-AP	Download protocol
WB protocol for ATP5C1 antibody 10910-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Autophagy AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells. Authors - Zhenhong Ni View Article
mouse	WB	Acta Pharm Sin B Cannabidiol alleviates methamphetamine addiction via targeting ATP5A1 and modulating the ATP-ADO-A1R signaling pathway. Authors - Sha Jin View Article
human	IF	Adv Sci (Weinh) Single-Mitochondrion ATP Profiling Directs Discovery of Targetable OXPHOS Dependency in Cancers. Authors - Xu Xiao View Article
mouse,human	WB	Adv Sci (Weinh) Icaritin Ameliorates Cisplatin-Induced Mitochondrial Metabolic Dysfunction-Associated Nephrotoxicity and Synergistically Potentiates Its Antitumor Efficacy. Authors - Piao Luo View Article
mouse	WB,IHC,IF	Sci Adv CHCHD2 mutant mice link mitochondrial deficits to PD pathophysiology. Authors - Szu-Chi Liao View Article
		J Exp Med Viral proteases activate the CARD8 inflammasome in the human cardiovascular system Authors - Rhea Nadkarni View Article

Validation Data Gallery

WB analysis using 10910-1-AP

WB analysis of HeLa using 10910-1-AP

WB analysis of mouse brain using 10910-1-AP

WB analysis of human heart using 10910-1-AP

WB analysis of human skeletal muscle using 10910-1-AP

IHC staining of human liver cancer using 10910-1-AP

IHC staining of human liver cancer using 10910-1-AP

IF Staining of HepG2 using 10910-1-AP

IF Staining of HepG2 using 10910-1-AP

IF Staining of HepG2 using 10910-1-AP

ATP5C1 Polyclonal antibody

ATP5C1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

AKT-mediated phosphorylation of ATG4B impairs mitochondrial activity and enhances the Warburg effect in hepatocellular carcinoma cells.

Cannabidiol alleviates methamphetamine addiction via targeting ATP5A1 and modulating the ATP-ADO-A1R signaling pathway.

Single-Mitochondrion ATP Profiling Directs Discovery of Targetable OXPHOS Dependency in Cancers.

Icaritin Ameliorates Cisplatin-Induced Mitochondrial Metabolic Dysfunction-Associated Nephrotoxicity and Synergistically Potentiates Its Antitumor Efficacy.

CHCHD2 mutant mice link mitochondrial deficits to PD pathophysiology.

Viral proteases activate the CARD8 inflammasome in the human cardiovascular system

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