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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP4B Polyclonal antibody

ATP4B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18692-1-AP

Synonyms

ATP4B, ATP6B, H+/K+ ATPase beta, Proton pump beta chain

Validation Data Gallery

Applications:
  • WB analysis using 18692-1-AP

    WB analysis using 18692-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse colon using 18692-1-AP

    WB analysis of mouse colon using 18692-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of rat kidney using 18692-1-AP

    WB analysis of rat kidney using 18692-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 18692-1-AP (ATP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse stomach tissue, mouse colon tissue, rat kidney tissue, rat stomach

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Sample-dependent, check data in validation data gallery

Product Information

18692-1-AP targets ATP4B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP4B fusion protein Ag6935 相同性解析による交差性が予測される生物種
Full Name ATPase, H+/K+ exchanging, beta polypeptide
Calculated molecular weight 33 kDa
Observed molecular weight 60-80 kDa
GenBank accession numberBC029059
Gene symbol ATP4B
Gene ID (NCBI) 496
RRIDAB_10597241
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ATP4B antibody 18692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols