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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ATP2B3/PMCA3 Polyclonal antibody (31692-1-AP)

    WB analysis using 31692-1-AP (same clone as 31692-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31692-1-AP (ATP2B3/PMCA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31692-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ATP2B3/PMCA3 Polyclonal antibody (31692-1-AP)

    WB analysis using 31692-1-AP (same clone as 31692-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31692-1-AP (ATP2B3/PMCA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31692-1-PBS in a different storage buffer formulation.

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ATP2B3/PMCA3 Polyclonal antibody, PBS Only

ATP2B3/PMCA3 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 31692-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31692-1-PBS targets ATP2B3/PMCA3 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP2B3/PMCA3 fusion protein Ag35923 相同性解析による交差性が予測される生物種
Full Name ATPase, Ca++ transporting, plasma membrane 3
Observed molecular weight 134 kDa
GenBank accession numberBC047580
Gene Symbol ATP2B3
Gene ID (NCBI) 492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ16720
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ATP2B3 also known as PMCA3, belongs to the family of P-type primary ion transport ATPases. ATP2B3 uses ATP as an energy source to transport cytosolic Ca2+ ions across the plasma membrane to the extracellular compartment. ATP2B3 is highly expressed in the brain and cerebellum and is important in regulating neuronal Ca2+. Mutations in ATP2B3 are associated with X-linked spinocerebellar ataxia-1 (PMID: 25953895, 22912398).