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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using ATP2B1 Recombinant antibody (83827-3-RR)

    WB analysis of mouse brain using 83827-3-RR (same clone as 83827-3-PBS)

    unboiled mouse brain tissue were subjected to SDS PAGE followed by western blot with 83827-3-RR (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse small intestine tissue using ATP2B1 Recombinant antibody (83827-3-RR)

    IHC staining of mouse small intestine using 83827-3-RR (same clone as 83827-3-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 83827-3-RR (ATP2B1 antibody) at dilution of 1:0 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83827-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83827-3-PBS

    Affinity and Kinetic Characterization of 83827-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83827-3-PBS against Human ATP2B1 was performed. The affinity constant is 4.46 nM.

  • Affinity and Kinetic Characterization of 83827-3-RR

    Affinity and Kinetic Characterization of 83827-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83827-3-RR against Human ATP2B1 were performed. The affinity constant is 10.3 nM.

ATP2B1 Recombinant monoclonal antibody, PBS Only

ATP2B1 Uni-rAb® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA
Cat No. 83827-3-PBS
Clone No.240848D10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

240848D10, PMCA1, PMCA1kb

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83827-3-PBS targets ATP2B1 in WB, IHC, Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag32423

Product name: Recombinant human ATP2B1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2-89 aa of NM_001001323.1

Sequence: GDMANNSVAYSGVKNSLKEANHDGDFGITLAELRALMELRSTDALRKIQESYGDVYGICTKLKTSPNEGLSGNPADLERREAVFGKNF

相同性解析による交差性が予測される生物種
Full Name ATPase, Ca++ transporting, plasma membrane 1
Calculated molecular weight135 kDa
Observed molecular weight130 kDa
GenBank accession numberNM_001001323.1
Gene Symbol ATP2B1
Gene ID (NCBI) 490
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP20020
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).