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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ATP2B1 Polyclonal antibody

ATP2B1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30035-1-AP

Synonyms

ATP2B1, PMCA1, PMCA1kb

Validation Data Gallery

Applications:
  • WB analysis using 30035-1-AP

    WB analysis using 30035-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30035-1-AP (ATP2B1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 30035-1-AP

    IHC staining of human colon using 30035-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30035-1-AP (ATP2B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP2B1 antibody included
  • IHC staining of human pancreas cancer using 30035-1-AP

    IHC staining of human pancreas cancer using 30035-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30035-1-AP (ATP2B1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP2B1 antibody included

Tested Applications

Positive WB detected in37°C incubated mouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ATP2B1 Ready-To-Use IHC Kit. ATP2B1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

30035-1-AP targets ATP2B1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATP2B1 fusion protein Ag32423 相同性解析による交差性が予測される生物種
Full Name ATPase, Ca++ transporting, plasma membrane 1
Calculated molecular weight135 kDa
Observed molecular weight130-180 kDa
GenBank accession numberNM_001001323.1
Gene symbol ATP2B1
Gene ID (NCBI) 490
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ATPase plasma membrane Ca2+ transporting 1 (ATP2B1, also known as plasma membrane Ca2+ pump isoform 1; PMCA1) belongs to the family of ATP-driven calmodulin-dependent Ca2+ pumps that participate in the regulation of intracellular free Ca2+ (PMID:35358416). The ATP2B1 contents of extracellular vesicles are increased in prostate cancer treated with enzalutamide and are negatively regulated by androgen receptors (PMID:29105980). ATP2B1 plays an important role in the prognosis of cholangiocarcinoma. Furthermore, ATP2B1 plays an important role in the prognosis of cholangiocarcinoma and can be a prognostic factor for cholangiocarcinoma (PMID:35875160).

Protocols

Product Specific Protocols
WB protocol for ATP2B1 antibody 30035-1-APDownload protocol
IHC protocol for ATP2B1 antibody 30035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols