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Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human ATP1B3 Rabbit Recombinant Antibody (98589-1-RR)

    FC experiment of human peripheral blood leukocytes using 98589-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human ATP1B3 Rabbit RecAb (98589-1-RR, Clone:251377D4) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human ATP1B3 Rabbit Recombinant Antibody (98589-1-RR)

    FC experiment of human peripheral blood leukocytes using 98589-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human ATP1B3 Rabbit RecAb (98589-1-RR, Clone:251377D4)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9)(blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity of 98589-1-RR

    Affinity of 98589-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98589-1-RR against Human ATP1B3 were performed. The affinity constant is below 1 pM.

Anti-Human ATP1B3 Rabbit Recombinant Antibody

ATP1B3 Uni-rAb® Recombinant Antibody for FC
Cat No. 98589-1-RR
Clone No.251377D4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

ATPB 3, ATPB-3, CD298, Sodium/potassium-dependent ATPase subunit beta-3, Sodium/potassium-transporting ATPase subunit beta-3

Formulation:  PBS, Azide
PBS, Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive FC detected inFC receptor blocked human peripheral blood leukocytes

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98589-1-RR targets ATP1B3 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg4947

Product name: Recombinant Human ATP1B3 protein (rFc Tag)

Source: mammalian cells-derived, pHZ-KIsec-N-rFc

Tag: N-rFc

Domain: 57-279 aa of BC011835

Sequence: TMWVMLQTLNDEVPKYRDQIPSPGLMVFPKPVTALEYTFSRSDPTSYAGYIEDLKKFLKPYTLEEQKNLTVCPDGALFEQKGPVYVACQFPISLLQACSGMNDPDFGYSQGNPCILVKMNRIIGLKPEGVPRIDCVSKNEDIPNVAVYPHNGMIDLKYFPYYGKKLHVGYLQPLVAVQVSFAPNNTGKEVTVECKIDGSANLKSQDDRDKFLGRVMFKITARA

相同性解析による交差性が予測される生物種
Full Name ATPase, Na+/K+ transporting, beta 3 polypeptide
Calculated molecular weight 32 kDa
GenBank accession numberBC011835
Gene Symbol ATP1B3
Gene ID (NCBI) 483
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP54709
Storage Buffer PBS with 0.09% sodium azide{{ptg:BufferTemp}}7.3
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

ATP1B3 (also known as CD298) is the β3 subunit of Na+/K+-ATPase which functions to maintain sodium and potassium gradients across membranes involved in cellular activities. ATP1B3 is a glycosylated protein and there are fully and intermediately glycosylated forms of ATP1B3 in mammalian cells. The predicted MW of ATP1B3 is around 32 kDa, while various forms (38-43 kDa) can be observed due to the different level of glycosylation (PMID: 30792309, 16339171, 17176442).

Protocols

Product Specific Protocols
FC protocol for ATP1B3 antibody 98589-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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